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Serov A. Ye., Tishkov V. I.

Role of the Proline Remains in Stability of Procaryotic and Eucaryotic Formiate Dehydrogenases

Abstract

Mutagenesis of non-prolyl amino acid residues to proline is one of the popular methods to stabilize proteins. The influence of these mutations on the thermal stability of NAD+-specific format dehydrogenases (FDH; KC. 1.2.1.2) from methylotrofic bacterium and baker's yeast was studied. This stabilizaion method can not be used in the case of the bacterial FDH. Low stability of FDH from baker's yeast is probably provided by low content of proline residues in this enzyme. However, the G141P replacement did not stbilize the yeast FDH. Unlike the bacterial enzyme, FDH from baker's yeast is possible to be stabilized by increasing rigidity of its polypeptide chain.
Moscow University Chemistry Bulletin.
2002, Vol. 43, No. 6, P. 345
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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