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Jose L. Martinez H., Iliyna A., Dominguez Malfavon L., Olga Sanchez C., Dustet M. J. C.

Partial characterization of penicillin acylase from fungi Aspergillus fumigatus and Mucor gryseocianum

Abstract

Preparation of Penicillin G acylase were obtained using two sources of fungus, Aspergillus fumigatus H/6.17.3and Mucor griseocyanum H/55.1: The fungi were propagated in Skim milk medium at 30ºC as the sole nitrient sorce and D‑Phenylglycine methyl ester as inducer. Dialyzed medium containing enzyme penicillin G acylase of Mucor gryseocianum and Aspergillus fumigatus was used. The phenylmethylsulfonyl fluoride was found to inhibit Penicillin G acylase activity of obtained preparations. The optimum pH range for the dialyzed preparations was pH 7-8 and pH 7.5- 8.5 respectively and optimum temperature for maximal enzyme activity of both sources, was at 40 ºC. Km value determined using the penicillin G as sustrate of enzymes of Mucor gryseocianum and Aspergillus fumigatus was 1.77 x 10-7 M and 1.46 x 10-7 M, respectively.
Moscow University Chemistry Bulletin.
2003, Vol. 44, No. 1, P. 53
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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