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Ilyina A., Hernández Vázquez M. G., Valdez Lara E. L., Chorne Navia R., Chávez Chávez R., Rodríguez Martínez J.

Prostaglandin H synthase activity in the presence of calcium ions and mouse skin in vitro

Abstract

Using preparation of microsomes obtained from bovine vesicular gland, the prostaglandin H synthase (PGHS, EC 1.14.99.1) activity was studied at a wide range of Ca2+ concentrations. It was demonstrated that at calcium concentrations lower than 0.005 mM, the activity was not changed significantly. Increase in calcium concentration resulted in enzyme inhibition and at the same time decreased the constant of enzyme inactivation during the reaction. The storage stability of lyophilized enzyme preparations obtained with and without calcium ions was evaluated. The effect of calcium ions to stabilize the PGHS, reported early for the enzyme solutions, was not observed. Moreover, the PGHS activity and PGE1 as well as PGE2 concentration were monitored in the presence of mice skin (1 cm2) applied as piece or in the homogenized form. It was observed that preincubation with skin at 37°C for more than 54 minutes slowly decreased the enzyme activity and prostaglandin concentration.
Moscow University Chemistry Bulletin.
2003, Vol. 44, No. 1, P. 57
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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