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A. E. Serov, V. I. Tishkov

Baker’s yeast formate dehydrogenase: unusual mechanism of inactivation and stabilization by ionic srength and cofactor

Abstract

Mechanism of thermal inactivation of baker’s yeast formate dehydrogenase (SceFDH) is uniquie for the family of NAD+-dependent FDHs. The enzyme inactivates in two stages with the first one being reversible, while the denaturation of other FDHs is described by non-reversible first-order kinetics. Unusual mechanism of inactivation and low thermal stability of SceFDH are most probably connected with the flexibility of its polypeptide chain. SceFDH is observed to be highly stabilyzied by ionic strength and cofactor.
Moscow University Chemistry Bulletin.
2006, Vol. 47, No. 2, P. 79
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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