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N. S. Teryoshina, A. A. Abramov, A. A. Markarian
Chromatographic analysis of
the berberis homoeopatic preparations
Abstract
Procedures of determination
of alkaloids of Berberis vulgaris in the matrix homoeopathic tincture and in
the multi component homoeopathic preparations have been developed. Composition
of tincture containing alkaloids has been studied by HPLC with help of diode
array multi-wavelength detector. 13 compounds have been revealed and the purity
of their peaks in tridimensional coordinates (time – intensity of absorption –
wavelength) has been determined. Quantitative estimation of total alkaloids in
the tincture (from 0.20 to 0.60 %) has been fulfilled. The content of berberine
from this amount is about 38–42 %. Methods of chromatographic “finger prints”
for determination of identity of homoeopathic preparation of Berberis are
disclosed.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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