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L. Yu. Filatova, N. L. Klyachko
PlyC
lysin - the enzyme active towards group A, C and E Streptococci: peculiarities
of inactivation and stabilization by intramolecular chemical cross linking
Abstract
Investigation of thermal inactivation of PlyC – the enzyme, lysing
group A, C and E streptococci, was carried out. As found, at 37–41.5°C, pH 6–8, and enzyme
concentration of 0.3–160 µg/ml inactivation of PlyC lysin found to be
oligomeric enzyme is caused by dissociation of its molecule. At higher (42–50°C) and lower (35–22°C) temperatures PlyC
inactivation is connected with monomolecular mechanism. The effective method of
PlyC stabilization by means of intramolecular chemical cross linking was
proposed.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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