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S. V. Uglanova, M. V. Popov, V. S. Kurova, E. V. Batrakova, D. Manickam, A. V. Kabanov, N. L. Klyachko

Stabilization of enzymes-antioxidants by complex- and conjugate formation with block-copolymers: prospects to cns treatment

Abstract

Nano-sized particles with radius of 16±2 nm based on complexes and conjugates of highly active superoxide dismutase and catalase with polyelectrolyte block copolymer poly(ethyleneimine)-poly(ethylene glycol) and similar bienzyme systems were obtained. Mass-spectrometry was used to confirm cross-linking of enzyme and block copolymer molecules in nanoparticles formed. Significant increase of SOD and catalase stability (up to 4 times) towards proteolytic degradation under chymotrypsin and trypsin action for 3 h at 37C was revealed when enzyme-containing nanoparticles were used for experiment. Antioxidant enzymes-containing polyelectrolyte nanoparticles are seemed to be prospect for BBB penetration and CNS drug delivery.
Moscow University Chemistry Bulletin.
2010, Vol. 51, No. 3, P. 227
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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