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L. I. Oleynik, T. S. Buslova, I. A. Veselova, T. N. Shekhovtsova
Kinetics of
quercetin oxidation catalyzed by horseradish peroxidase in the presence of b-cyclodextrin
Abstract
The rate of quercetin oxidation catalyzed by
horseradish peroxidase was found to increase by 20% in the presence of
macrocyclic complexing agent b-cyclodextrin. The
comparison of the kinetic parameters of this reaction in the presence and in
the absence of b-cyclodextrin showed that the introduction of b-cyclodextrin
did not influence significantly the specificity of the enzyme towards the
reducing substrate (estimated as kcat/Km ratio). At
the same time, the reaction rate did not depend on the time of incubation of
quercetin and b-cyclodextrin. The increase of the
reaction rate was supposed to be associated with non-specific interaction
between b-cyclodextrin and the product of quercetin
oxidation.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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