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N. G. Balabushevich, M. A. Pechenkin, A. V. Lopes de Gerenyu, I. N. Zorov, E. V. Mikhalchik, N. I. Larionova
Layer-by-layer adsorption of
biopolyelectrolytes as a universal approach to fabrication of protein-loaded
microparticles
Abstract
The study was aimed at examination of the
microparticles formed via the layer-by-layer adsorption of dextran sulfate and
chitosan onto the insoluble complexes of various proteins with polyanion.
Microparticles with all tested proteins were stable at pH 1–5. At pH > 6 the
mucoadhesivity of the microparticles changed and the encapsulated proteins
released. Microparticles were able to protect the proteins from proteases.
Protein protease inhibitors encapsulated as well (2–3%) completely prevented
the proteins proteolysis. The pharmacological effect of microencapsulated
insulin was studied in vivo in the model of chronic diabetes in rats
treated by its oral administration.
Key words: мicroencapsulation, layer-by-layer biopolyelectrolyte adsorption,
proteins, proteolysis, protein delivery.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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