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I. A. Shanin, NguyenThiDieuThuy, S. A. Eremin
Determination of levofloxacin (levorotatory
stereoisomer of ofloxacin) in milk by indirect enzyme-linked immunosorbent
assay
Abstract
In this work we
were obtained polyclonal antibodies for levofloxacin. We synthesize conjugates
of levofloxacin with cationized BSA for immunization and with ovalbumin for
development of ELISA for the detection of levofloxacin. The developed method is
characterized by range of detectable concentrations of 0,03 ng/ml to 0,41 ng /
mL, and the limit of detectable concentrations – 0,01 ng/ml. We tested the 28
fluoroquinolones for cross reactivity and only ofloxacin (145%), marbofloxacin
(82%), ofloxacin dextrorotatory form (68%), rufloxacin (67%), garenoxacin (24%)
has cross reactivity. Optimized ELISA technique allows
the detection of levofloxacin in milk from 0,33 ng/ml to 3,34 ng/ml. The
recoveries were in the range of 89,5–102% with a relative standard deviation of
3%. We tested 45 real samples of milk purchased in a local stores, for 5 of
them result were positive (near 1 ng/ml).
Key words: ELISA, conjugate with protein,
fluoroquinolones, ofloxacin, levofloxacin, milk.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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