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T. O. Samarina, M. K. Beklemishev

Determinationof amikacin by rayleigh scattering method based on the covalent bonding of the analyte with a water soluble polymer

Abstract

A method for the determination of aminoglycoside antibiotics based on the covalent bonding of the analyte with a water-soluble polymer by Rayleigh scattering method (λex = λem) is proposed. Determination of amikacin (as model analyte) is carried out by the carbodiimide crosslinking technique that forms amide bonds between the co-polymer of 4-styrenesulfonic acid and maleic acid and primary amine groups of amikacin. Conditions of the reaction have been selected to obtain maximum scattering intensity of the product: reaction time 15 min, pH 7.0, ionic strength 0.02, measuring of scattering intensity at 362 nm. The determination of amikacin is not influenced by (mol/mol ratio): Al(III), Co(II), Cu(II), Fe(III), Ni(II), Zn(II) – 300, Cl and NO3– 250, Ca(II) and Mg(II) – 50, HCO3 – 20; nitrogen compounds affect at 20 – 50-fold, protein – 2.5-fold ratio. The calibration plot was constructed over the range of 0.2– 35 mg/L (r = 0.993), LOD(3s-criterium) is 0.08 mg/L (n = 10, P = 0.95). The analyses on the drug powder for injection and gel have been performed.
Key words: Rayleigh scattering method, aminoglycosides, amikacin, carbodiimide cross-linking technique, drug analysis.
Moscow University Chemistry Bulletin.
2015, Vol. 56, No. 5, P. 279
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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