|
D. A. Matolygina, H. E. Osipova, S. A. Smirnov, N. G. Belogurova, N. L. Eremeev, V. I. Tishkov, A. V. Levashov, P. A. Levashov
Determination
of bacteriolytic activity and measurement of enzyme sorption by cells in the
system of living Lactobacillus plantarum
Abstract
It has been developed turbidimetric method for determining the activity
of bacteriolytic enzymes with live cells Lactobacillus plantarum as substrate. Measuring method for
enzyme sorption on cells under same conditions is described. Chicken egg
lysozyme was used as the standard model enzyme. The correctness of the
calculations of kinetic parameters is proved by counting of colony forming
units (CFU). The physicochemical parameters of enzyme adsorption on cells are
calculated and the correctness of the obtained equilibrium constants of
desorption is verified.
Key words: chicken egg lysozyme, Lactobacillus plantarum, bacteriolytic
activity.
Copyright (C) Chemistry Dept., Moscow State University, 2002
|
|
